The
benefits of time, or pulsed, methods are now available
on the FluoroLog®,
in addition to the long established frequency domain.
For the method that fits your experiments best, you
have only one place to go HORIBA Jobin Yvon.
You can add a new dimension in molecular dynamics to your Fluorolog® with Time Correlated Single Photon Counting. With the unification of IBH and SPEX® products, you can now add the versatility and ultimate sensitivity of TCSPC to the most sensitive spectrofluorometer in the world, the FluoroLog®-3. Choose the broad wavelength range of a flashlamp for excitation, or the speed and power of a laser diode or LED from a NanoLED source. No matter what you select, you always have the modular flexibility and years of experience built into the Fluorolog®-3 as well as the TCSPC expertise that has made IBH the leader in time domain acquisition.
TCSPC measurements use a pulsed light source and a circuit to detect single photon fluorescence at a detector. In a repetitive series of many start stop signals from the circuitry, a binned histogram in time channels of single photon counts is gradually generated. Fluorescence lifetimes can be obtained in seconds to minutes with repetition rates in the MHz range. In addition, the decay statistics are Poissonian, allowing the data to be rigorously analyzed. TCSPC is compatible with our NanoLED series of precise and inexpensive pulsed light emitting diodes and diode laser sources. The single photon detection method is preferred when photon emission is too weak for frequency domain detection.
Along with steady state experiments, such as emission, excitation, and synchronous scans, all standard lifetime scans are possible on the new Fluorolog®-3 with TCSPC capability, for example, lifetime, dynamic anisotropy, time resolved spectra, etc. We replace the standard R928 photomultiplier tube with the HORIBA Jobin Yvon IBH TBX-04 detector. Besides the solid state NanoLED sources from near-UV to near-IR (available in selected wavelengths from 375 to 830 nm), an intense, broadband 450-W CW xenon lamp, and the HORIBA Jobin Yvon IBH 5000F coaxial nanosecond flashlamp can be attached. Options include extended red and thermoelectrically cooled detection, as well as phosphorimeter for long lifetime luminescence measurements, temperature baths, microwell plate reader, automated polarizers, and more.
Pulsed light source (gray) and response (black) of the sample, POPOP, showing the gradual decay of fluorescence intensity with time. A single exponential fit (dotted red) gives a lifetime of 1.309 ± 0.003 ns.
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